Protein Quality Evaluation Methods

Biological Value (%) is calculated as retained nitrogen/gain/protein intake and, although a useful measure
absorbed nitrogen X 100. To illustrate how thiswhen used in growing animals, has little value for
provides an evaluation of protein quality, consider ahumans for two reasons. First, this method is not used
scenario in which all amino acids in a protein are usedin human experiments because, ethically, human
for protein synthesis. Here, retained and absorbedsubjects cannot be deprived of necessary nutrients.
nitrogen would be equal and the biological value wouldSecond, the difference in species growth rates, Le.,
equal 100%.lower in humans versus rats, results in errors when
Conversely, if some indispensable amino acids are notanimal data are used to estimate human needs.
available in the protein source, protein synthesis isProtein Digestibility-Corrected Amino Acid Score is
impaired and some unused nitrogen is excreted frombased on the amino acid content of a food corrected
the body, Le., retained nitrogen decreases relative tofor digestibility. The score reflects the limiting
absorbed and the biological value drops below 100%.indispensable amino acids (those amino acids that
Consequently, some foods such as complete proteinhumans cannot synthesize) in a food. The reference
foods (e.g., milk) have high biological values (95%), whilevalue used is the requirement for a 2-5-year-old
incomplete protein foods are much lower (Le., cornindividual. This provides a built-in safety buffer because
[60%1). It is not possible to have a biological value > 1protein needs of this age group are greater than for
00%. Net Protein Utilization is a similar measure butadults.
involves a direct measure of retained nitrogen, Le.,About information on bodybuilding & fitness . Also did
destruction and analysis of the body, and therefore isyou know about conditions & diseases and types of
only used with animal studies.natural supplement .
Protein Efficiency Ratio is determined as body mass